Article
Spontaneous mutations in the human gene for p53 in recombinant adenovirus during multiple passages in human embryonic kidney 293 cells.
Biochemical and biophysical research communications - 10 Jan 2003
Ugai Hideyo, Suzuki Erika, Inabe Kumiko, Murata Takehide, Hamada Hirofumi, Yokoyama Kazunari K
Abstract excerpt
Infectious recombinant adenovirus (rAd) is usually produced in human embryonic kidney 293 cells that harbor the E1 gene and rAd has been shown to be an efficient tool for gene transfer both in vivo and in vitro. It also has considerable potential in human gene therapy. However, rates of spontaneous mutations in genes introduced into host cells after multiple passages remain to be clarified. We have characterized...
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