Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Degenerated pIX-IVa2 adenoviral vector sequences lowers reacquisition of the E1 genes during virus amplification in 293 cells.

Gene therapy - 1 Nov 2001

Robert J J, Gauffeny I, Maccario J, Jullien C, Benoit P, Vigne E, Crouzet J, Perricaudet M, Yeh P

Abstract excerpt

A critical issue for E1-deleted adenoviral vectors manufactured from 293 cells is the emergence of replication-competent adenovirus (RCA). These contaminants arise through homologous recombination between identical sequences framing the E1 locus displayed by 293 cells, and the vector backbones. Modified recombinogenic sequences (syngen) were thus introduced within the vector backbone, and virus viability and RCA...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Adenoviridae
Share to Cell Line

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Gene Deletion

Share to Genes, p53

Share to Genetic Engineering

Share to Genetic Vectors

Share to Tumor Cells, Cultured

Share to Virus Replication

Source

Identifiers

PMID: 11892839
DOI: 10.1038/sj.gt.3301562

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Degenerated pIX-IVa2 adenoviral vector sequences lowers reacquisition of the E1 genes during virus amplification in 293 cells.PMID 11892839Read ↗

Molecular characterization of replication-competent variants of adenovirus vectors and genome modifications to prevent their occurrence.Semantic proximity Read ↗

Characterization of Replication-Competent Adenovirus Isolates from Large-Scale Production of a Recombinant Adenoviral VectorSemantic proximity Read ↗

Adenoviral Producer CellsSemantic proximity Read ↗

A gene transfer vector-cell line system for complete functional complementation of adenovirus early regions E1 and E4Semantic proximity Read ↗

The X Gene of Adeno-Associated Virus 2 (AAV2) Is Involved in Viral DNA ReplicationSemantic proximity Read ↗

"Hit-and-run" transformation by adenovirus oncogenes.Semantic proximity Read ↗

Frequency and Stability of Chromosomal Integration of Adenovirus VectorsSemantic proximity Read ↗

A Single Short Stretch of Homology Between Adenoviral Vector and Packaging Cell Line Can Give Rise to Cytopathic Effect-Inducing, Helper-Dependent E1-Positive ParticlesSemantic proximity Read ↗

Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulationSemantic proximity Read ↗

Genetic analysis of adenovirus E1A: induction of genetic instability and altered cell morphologic and growth characteristics are segregatable functions.Semantic proximity Read ↗

Spontaneous mutations in the human gene for p53 in recombinant adenovirus during multiple passages in human embryonic kidney 293 cells.Semantic proximity Read ↗

Integrated viral genomes can be lost from adenovirus type 12-induced hamster tumor cells in a clone-specific, multistep process with retention of the oncogenic phenotype.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.