Article
B cell development arrest upon insertion of a neo gene between JH and Emu: promoter competition results in transcriptional silencing of germline JH and complete VDJ rearrangements.
Journal of immunology (Baltimore, Md. : 1950) - 15 Dec 2002
Delpy Laurent, Decourt Catherine, Le Bert Marc, Cogné Michel
Abstract excerpt
Previous targeting experiments within the IgH locus have shown that V(D)J recombination was affected by an insertion of a neo gene within E(mu) upstream of the core enhancer, but not by insertions downstream of the enhancer. Similarly, class switch recombination to a given (C) gene was affected only by interposition of neo in between that gene and the 3' IgH enhancers. Here we show that insertion of neo upstream...
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