Article
Mutations of the intronic IgH enhancer and its flanking sequences differentially affect accessibility of the JH locus.
The EMBO journal - 1 Dec 1993
Chen J, Young F, Bottaro A, Stewart V, Smith R K, Alt F W
Abstract excerpt
To investigate the role of intronic immunoglobulin heavy chain (IgH) enhancer (E mu) in generating accessibility of the JH locus for VDJ recombination, we generated ES cells in which E mu or its flanking sequences were mutated by replacement with or insertion of an expressed neor gene. Heterozygo...
Topics
- Animals
- Cell Line, Transformed
- Enhancer Elements, Genetic
- Gene Rearrangement, B-Lymphocyte, Heavy Chain
- Immunoglobulin Heavy Chains
- Immunoglobulin Joining Region
- Introns
- Methylation
- Mice
- Mutation
- Restriction Mapping
- Transcription, Genetic