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Article

Mutations of the intronic IgH enhancer and its flanking sequences differentially affect accessibility of the JH locus.

The EMBO journal - 1 Dec 1993

Chen J, Young F, Bottaro A, Stewart V, Smith R K, Alt F W

Abstract excerpt

To investigate the role of intronic immunoglobulin heavy chain (IgH) enhancer (E mu) in generating accessibility of the JH locus for VDJ recombination, we generated ES cells in which E mu or its flanking sequences were mutated by replacement with or insertion of an expressed neor gene. Heterozygous mutant ES cells were used to generate chimeric mice from which pre-B cell lines were derived by transformation of...

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Identifiers

PMID: 8223473
DOI: 10.1002/j.1460-2075.1993.tb06152.x
PMCID: PMC413901

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Mutations of the intronic IgH enhancer and its flanking sequences differentially affect accessibility of the JH locus.PMID 8223473Read ↗

B cell development arrest upon insertion of a neo gene between JH and Emu: promoter competition results in transcriptional silencing of germline JH and complete VDJ rearrangements.Semantic proximity Read ↗

Elucidation of IgH intronic enhancer functions via germ-line deletionSemantic proximity Read ↗

Recombination may occur in the absence of transcription in the immunoglobulin heavy chain recombination centre.Semantic proximity Read ↗

Gene-targeted deletion and replacement mutations of the T-cell receptor beta-chain enhancer: the role of enhancer elements in controlling V(D)J recombination accessibility.Semantic proximity Read ↗

Antisense intergenic transcription precedes Igh D-to-J recombination and is controlled by the intronic enhancer Emu.Semantic proximity Read ↗

Deletion of the immunoglobulin kappa chain intron enhancer abolishes kappa chain gene rearrangement in cis but not lambda chain gene rearrangement in trans.Semantic proximity Read ↗

A role for the IgH intronic enhancer E mu in enforcing allelic exclusion.Semantic proximity Read ↗

Promoter Proximity Defines Mutation Window for VH and VΚ Genes Rearranged to Different J Genes.Semantic proximity Read ↗

Regulation of IgH gene assembly: role of the intronic enhancer and 5'DQ52 region in targeting DHJH recombination.Semantic proximity Read ↗

Requirement for enhancer specificity in immunoglobulin heavy chain locus regulation.Semantic proximity Read ↗

The IgH <i>Eμ</i> -MAR regions promote UNG-dependent error-prone repair to optimize somatic hypermutationSemantic proximity Read ↗

The IgH Eµ-MAR regions promote UNG-dependent error-prone repair to optimize somatic hypermutation.Semantic proximity Read ↗

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