Article
Changing the net charge from negative to positive makes ribonuclease Sa cytotoxic.
Protein science : a publication of the Protein Society - 1 Oct 2002
Ilinskaya Olga N, Dreyer Florian, Mitkevich Vladimir A, Shaw Kevin L, Pace C Nick, Makarov Alexander A
Abstract excerpt
Ribonuclease Sa (pI = 3.5) from Streptomyces aureofaciens and its 3K (D1K, D17K, E41K) (pI = 6.4) and 5K (3K + D25K, E74K) (pI = 10.2) mutants were tested for cytotoxicity. The 5K mutant was cytotoxic to normal and v-ras-transformed NIH3T3 mouse fibroblasts, but RNase Sa and 3K were not. The structure, stability, and activity of the three proteins are comparable, but the net charge at pH 7 increases from -7 for...
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