Article
A nuclear localization sequence endows human pancreatic ribonuclease with cytotoxic activity.
Biochemistry - 2 Mar 2004
Bosch Montserrat, Benito Antoni, Ribó Marc, Puig Teresa, Beaumelle Bruno, Vilanova Maria
Abstract excerpt
Some members of the ribonuclease superfamily, such as Onconase, are cytotoxic to cancer cells. This is not the case for human pancreatic ribonuclease. This lack of cytotoxicity is probably a result of the inhibition exerted by the cytosolic ribonuclease inhibitor once the protein has reached the cytosol. Until now, all cytotoxic human pancreatic ribonuclease variants have been described as being resistant to the...
Topics
- Active Transport, Cell Nucleus
- Catalysis
- Cell Nucleus
- Drug Design
- Endocytosis
- Enzyme Inhibitors
- Enzyme Stability
- Genetic Variation
- Growth Inhibitors
- HeLa Cells
- Humans
- Inhibitory Concentration 50
- Intracellular Fluid
- Isoenzymes
- K562 Cells
- Mutagenesis, Site-Directed
- Nuclear Localization Signals
- Ribonuclease, Pancreatic