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Article

Purification, kinetic and thermodynamic studies of a new ribonuclease from a mutant of Aspergillus niger.

Journal of biotechnology - 10 Oct 2005

Xiong Ya-Hong, Liu Jian-Zhong, Song Hai-Yan, Ji Liang-Nian

Abstract excerpt

Ribonuclease was purified from Aspergillus niger SA-13-20 to homogeneity level by using (NH(4))(2)SO(4) precipitation, DEAE-cellulose anion-exchange chromatography, ultrafiltration and Sephacryl HR-200 chromatography. The molecular weight and isoelectric point of the enzyme was 40.1kDa and 5.3, respectively. The pH- and temperature-dependent kinetic parameters were determined. The RNase showed the strongest...

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Identifiers

PMID: 15946756
DOI: 10.1016/j.jbiotec.2005.04.008

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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