Article
Purification and partial characterization of an extracellular ribonuclease from a mutant of Aspergillus niger.
Applied biochemistry and biotechnology - 1 Jun 2005
Xiong Ya-Hong, Liu Jian-Zhong, Song Hai-Yan
Abstract excerpt
A new extracellular ribonuclease (RNase) from a mutant of Aspergillus niger, named A. niger SA-13-20 RNase, was purified to homogeneity by (NH4)2SO4 fractionation (50-85%), DEAE-cellulose anion-exchange chromatography, ultrafiltration and Sephacryl HR-200 chromatography. The enzyme was purified up to 54.4-fold with a final yield of 24.5%. There were differences in the molecular weight, pI value and some...
Topics
- Aspergillus niger
- Enzyme Activation
- Enzyme Stability
- Extracellular Fluid
- Hydrogen-Ion Concentration
- Molecular Weight
- Mutation
- RNA
- Ribonucleases
- Species Specificity
- Temperature