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Article

Three-dimensional structure of a human pancreatic ribonuclease variant, a step forward in the design of cytotoxic ribonucleases.

Journal of molecular biology - 13 Oct 2000

Pous J, Canals A, Terzyan S S, Guasch A, Benito A, Ribó M, Vilanova M, Coll M

Abstract excerpt

We have determined the crystal structure of a human pancreatic ribonuclease or RNase 1 variant at 1.65 A resolution. Five residues in the N-terminal region were substituted by the corresponding amino acids of the bovine seminal RNase. In addition, a Pro to Ser mutation was present at position 50. The substitution of part of the N terminus has been critical both in improving the expression of this enzyme as a...

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PMID: 11021969
DOI: 10.1006/jmbi.2000.4506

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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