Article
Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi.
Current biology : CB - 6 Aug 2002
Simmer Femke, Tijsterman Marcel, Parrish Susan, Koushika Sandhya P, Nonet Michael L, Fire Andrew, Ahringer Julie, Plasterk Ronald H A
Abstract excerpt
RNA interference (RNAi) is a broadly used reverse genetics method in C. elegans. Unfortunately, RNAi does not inhibit all genes. We show that loss of function of a putative RNA-directed RNA polymerase (RdRP) of C. elegans, RRF-3, results in a substantial enhancement of sensitivity to RNAi in diverse tissues. This is particularly striking in the nervous system; neurons that are generally refractory to RNAi in a...
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