Article
Targeted deletion of mNth1 reveals a novel DNA repair enzyme activity.
Molecular and cellular biology - 1 Sept 2002
Ocampo Maria T A, Chaung Wenren, Marenstein Dina R, Chan Michael K, Altamirano Alvin, Basu Ashis K, Boorstein Robert J, Cunningham Richard P, Teebor George W
Abstract excerpt
DNA N-glycosylase/AP (apurinic/apyrimidinic) lyase enzymes of the endonuclease III family (nth in Escherichia coli and Nth1 in mammalian organisms) initiate DNA base excision repair of oxidized ring saturated pyrimidine residues. We generated a null mouse (mNth1(-/-)) by gene targeting. After almost 2 years, such mice exhibited no overt abnormalities. Tissues of mNth1(-/-) mice contained an enzymatic activity...
Topics
- Animals
- Apoptosis
- Apurinic Acid
- Carbon-Oxygen Lyases
- Crosses, Genetic
- DNA Methylation
- DNA Repair
- DNA-(Apurinic or Apyrimidinic Site) Lyase
- Deoxyribonuclease (Pyrimidine Dimer)
- Endodeoxyribonucleases
- Escherichia coli Proteins
- Female
- Gene Targeting