Article
Detection of site-specific proteolysis in secretory pathways.
Biochemical and biophysical research communications - 16 Aug 2002
Kim Sung Yun, Park Daeho, Oh Myungsok, Sellamuthu Saravanan, Park Woo Jin
Abstract excerpt
We report here a genetic assay suitable for detecting site-specific proteolysis in secretory pathways. The yeast enzyme invertase is linked to the truncated lumenal region of the yeast Golgi membrane protein STE13 via a protease substrate domain in a Saccharomyces cerevisiae strain lacking invertase. When the substrate is cleaved by a specific protease, the invertase moiety is released into the periplasmic space...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
