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Article

A yeast-based growth assay for the analysis of site-specific proteases.

Nucleic acids research - 15 Feb 2003

Köhler Fabian

Abstract excerpt

Many cellular and viral processes depend on site-specific proteolysis. Here, a genetic system for the identification of such proteases and inhibitors is described. The system utilizes the temperature- sensitive Saccharomyces cerevisiae CDC25-2 mutant strain and exploits the strict requirement of membrane localization of a constitutively active Ras mutant for the complementation of the yeast growth defect at the...

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Identifiers

PMID: 12582261
DOI: 10.1093/nar/gng016
PMCID: PMC150248

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Source receipt: pubmed, retrieved 7 Sept 2026.

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