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Article

Engineering of Kex2 variants exhibiting altered substrate specificity.

Biochemical and biophysical research communications - 2 Dec 2005

Han Hye-Eun, Rho Seong-Hwan, Lee Yong Jae, Park Woo Jin

Abstract excerpt

Engineering of secreted protease variants exhibiting altered substrate specificity is a challenging task because effective screening methods for the desired property are not available yet. In this study, we sought to obtain variants of Kex2, a yeast Golgi protease, which exhibit altered P2 specificity. We first randomly mutated three Asp residues (D176, D210, and D211) that constitute the S2 pocket of Kex2 and...

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Identifiers

PMID: 16229820
DOI: 10.1016/j.bbrc.2005.09.158

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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