Article
Functional expression of murine LRP1 requires correction of Lrp1 cDNA sequences.
Biochimica et biophysica acta - 19 Aug 2002
Smeijers Liesbet, Willems Stefan, Lauwers Annick, Thiry Els, van Leuven Fred, Roebroek Anton J M
Abstract excerpt
Here, we describe the reconstruction of a functional 14 kbp full-length murine Lrp1 cDNA from overlapping partial cDNAs, which were described before [Biochim. Biophys. Acta 1173 (1993) 71]. The reconstructed full-length cDNA needed sequence correction (by mutagenesis) due to nucleotide errors present in the underlying partial cDNAs. These mistakes compromised the proteolytical maturation of the LRP precursor...
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