Article
Mutations in conserved regions 1, 2, and 3 of Raf-1 that activate transforming activity.
Molecular carcinogenesis - 1 Apr 2002
Chan Edmond Y W, Stang Stacey L, Bottorff Drell A, Stone James C
Abstract excerpt
To investigate the role of Raf-1 in v-Ha-ras transformation, we have isolated and characterized a number of Raf-1 mutants that display increased transforming activity in Rat2 fibroblasts. A dipeptide deletion (Delta144-145) in the cysteine-rich domain (CRD) of conserved region (CR) 1 increased the interaction between Raf-1 and v-Ha-ras effector loop mutants in the yeast two-hybrid system, supporting the proposal...
Topics
- Alleles
- Amino Acid Sequence
- Amino Acid Substitution
- Animals
- Cell Line
- Cell Transformation, Neoplastic
- Conserved Sequence
- Fibroblasts
- Genes, ras
- MAP Kinase Signaling System
- Microscopy, Phase-Contrast
- Mitogen-Activated Protein Kinases
- Molecular Sequence Data
- Mutation
- Protein Serine-Threonine Kinases
- Proto-Oncogene Proteins c-raf
- Rats
- Recombinant Proteins