Article
Identification of discrete segments of human Raf-1 kinase critical for high affinity binding to Ha-Ras.
The Journal of biological chemistry - 9 Dec 1994
Ghosh S, Bell R M
Abstract excerpt
A critical event in a signal transduction pathway downstream of receptor tyrosine kinases is the physical association of GTP-liganded Ras with the serine/threonine kinase, Raf-1. The binding of Raf-1 to Ras results in translocation of the kinase to the plasma membrane and facilitates its activation by an unknown mechanism. A deletion mutagenesis approach was employed to elucidate critical sequences in Raf-1...
Topics
- Base Sequence
- DNA Primers
- Humans
- Molecular Sequence Data
- Mutation
- Protein Binding
- Protein Serine-Threonine Kinases
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-raf
- Sequence Deletion
- ras Proteins