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Article

Role of Raf-1 conserved region 2 in regulation of Ras-dependent Raf-1 activation.

Biochemical and biophysical research communications - 19 May 2000

Sendoh H, Hu C D, Wu D, Song C, Yamawaki-Kataoka Y, Kotani J, Okada T, Shima F, Kariya K, Kataoka T

Abstract excerpt

Full activation of Raf-1 requires the interaction of its CRD with Ras. The serine/threonine-rich region, CR2, of Raf-1 was implicated in Raf-1 regulation, but the underlying mechanism was unclear. Here we show that CRD loses its Ras-binding activity when expressed in connection with CR2, suggesting that CR2 masks CRD. This masking effect is abolished by substitution of Asp or Ala for Ser-259, a growth factor- and...

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Identifiers

PMID: 10814507
DOI: 10.1006/bbrc.2000.2674

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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