Inhibitors of Ras/Raf-1 interaction identified by two-hybrid screening revert Ras-dependent transformation phenotypes in human cancer cells.

doi:10.1073/pnas.222222699

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Article

Inhibitors of Ras/Raf-1 interaction identified by two-hybrid screening revert Ras-dependent transformation phenotypes in human cancer cells.

Proceedings of the National Academy of Sciences of the United States of America - 29 Oct 2002

Kato-Stankiewicz Juran, Hakimi Irina, Zhi Gang, Zhang Jie, Serebriiskii Ilya, Guo Lea, Edamatsu Hironori, Koide Hiroshi, Menon Sanjay, Eckl Robert, Sakamuri Sukumar, Lu Yingchun, Chen Quin-Zene, Agarwal Seema, Baumbach William R, Golemis Erica A, Tamanoi Fuyuhiko, Khazak Vladimir

Abstract excerpt

The interaction of activated Ras with Raf initiates signaling cascades that contribute to a significant percentage of human tumors, suggesting that agents that specifically disrupt this interaction might have desirable chemotherapeutic properties. We used a subtractive forward two-hybrid approach to identify small molecule compounds that block the interaction of Ras with Raf. These compounds (MCP1 and its...

Topics

3T3 Cells
Animals
CHO Cells
Cricetinae
Gene Library
Humans
Luciferases
MAP Kinase Signaling System
Mice

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mitogen-Activated Protein Kinases

Phenotype

Protein Binding

Proto-Oncogene Proteins c-raf

Serum Response Element

Transcription Factor AP-1

Transcriptional Activation

Transformation, Genetic

Tumor Cells, Cultured

Source

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Identifiers

PMID: 12391290
DOI: 10.1073/pnas.222222699
PMCID: PMC137895

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