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Article

Purification and crystallization of the yeast elongation factor eEF2.

Acta crystallographica. Section D, Biological crystallography - 1 Apr 2002

Jørgensen René, Carr-Schmid Anne, Ortiz Pedro A, Kinzy Terri Goss, Andersen Gregers Rom

Abstract excerpt

Crystals of the Saccharomyces cerevisiae elongation factor 2 (eEF2) in complex with GDP were obtained with the vapour-diffusion technique after rapid purification from industrial yeast. The crystals diffract to 2.85 A and belong to the space group P2(1)2(1)2(1). A yeast strain expressing a functional histidine-tagged eEF2 as the only form of the protein further allows facilitated purification of the factor for...

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PMID: 11914505
DOI: 10.1107/s0907444902003001

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