Article
Two-dimensional and epitaxial crystallization of a mutant form of yeast RNA polymerase II.
Journal of molecular biology - 5 Sept 1991
Darst S A, Kubalek E W, Edwards A M, Kornberg R D
Abstract excerpt
A mutant form of yeast RNA polymerase II that lacks the fourth and seventh largest subunits, referred to as pol II delta 4/7, crystallized on positively charged lipid layers. Both single-layered (two-dimensional) crystals and several multi-layered crystal forms were obtained. The two-dimensional crystals, preserved in negative stain, diffracted strongly to about 1/20 A-1 and more weakly to 1/13 A-1 resolution. A...
Topics
- Crystallization
- Microscopy, Electron
- Moire Topography
- Mutation
- RNA Polymerase II
- Saccharomyces cerevisiae
- X-Ray Diffraction