Article
Purification and properties of yeast ATP (CTP):tRNA nucleotidyltransferase from wild type and overproducing cells.
The Journal of biological chemistry - 25 Sept 1990
Chen J Y, Kirchner G, Aebi M, Martin N C
Abstract excerpt
ATP (CTP):tRNA nucleotidyltransferase (EC 2.7.7.25) has been purified from wild type cells of the yeast Saccharomyces cerevisiae, as well as from a strain that overproduces the activity. Purification from the wild type strain was accomplished with a multistep protocol including ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and affinity chromatography. The purified enzyme is near...
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