Article
Purification and properties of yeast ATP (CTP):tRNA nucleotidyltransferase from wild type and overproducing cells.
The Journal of biological chemistry - 25 Sept 1990
Chen J Y, Kirchner G, Aebi M, Martin N C
Abstract excerpt
ATP (CTP):tRNA nucleotidyltransferase (EC 2.7.7.25) has been purified from wild type cells of the yeast Saccharomyces cerevisiae, as well as from a strain that overproduces the activity. Purification from the wild type strain was accomplished with a multistep protocol including ammonium sulfate f...
Topics
- Chromatography
- Chromatography, Affinity
- Chromatography, Gel
- Chromatography, Ion Exchange
- Durapatite
- Hydroxyapatites
- Kinetics
- Molecular Weight
- Mutation
- RNA Nucleotidyltransferases
- Saccharomyces cerevisiae
