Article
In vitro study of two dominant inhibitory GTPase mutants of Escherichia coli translation initiation factor IF2. Direct evidence that GTP hydrolysis is necessary for factor recycling.
The Journal of biological chemistry - 5 Mar 1999
Luchin S, Putzer H, Hershey J W, Cenatiempo Y, Grunberg-Manago M, Laalami S
Abstract excerpt
We have recently shown that the Escherichia coli initiation factor 2 (IF2) G-domain mutants V400G and H448E do not support cell survival and have a strong negative effect on growth even in the presence of wild-type IF2. We have isolated both mutant proteins and performed an in vitro study of their main functions. The affinity of both mutant proteins for GTP is almost unchanged compared with wild-type IF2....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.