Article
Characteristics of brain Kv1 channels tailored to mimic native counterparts by tandem linkage of alpha subunits: implications for K+ channelopathies.
The Journal of biological chemistry - 10 May 2002
Akhtar Sobia, Shamotienko Oleg, Papakosta Marianthi, Ali Farooq, Dolly J Oliver
Abstract excerpt
Most neuronal Kv1 channels contain Kv1.1, Kv1.2 alpha, and Kvbeta2.1 subunits, yet the influences of their stoichiometries on properties of the (alpha)(4)(beta)(4) variants remain undefined. cDNAs were engineered to contain 0, 1, 2, or 4 copies of Kv1.1 with the requisite number of Kv1.2 and co-expressed in mammalian cells with Kvbeta2.1 to achieve "native-like" hetero-oligomers. The monomeric (Kv1.1 or 1.2),...
Topics
- Animals
- Binding Sites
- Blotting, Western
- Brain
- Cell Line
- Chromatography, Gel
- Cricetinae
- DNA, Complementary
- Dimerization
- Electrophoresis, Polyacrylamide Gel
- Electrophysiology
- Inhibitory Concentration 50
- Kinetics