Article
Mutational analysis of ganglioside GM(1)-binding ability, pentamer formation, and epitopes of cholera toxin B (CTB) subunits and CTB/heat-labile enterotoxin B subunit chimeras.
Infection and immunity - 1 Mar 2002
Jobling Michael G, Holmes Randall K
Abstract excerpt
Variants of cholera toxin B subunit (CTB) were made by bisulfite- and oligonucleotide-directed mutagenesis of the ctxB gene. Variants were screened by a radial passive immune hemolysis assay (RPIHA) for loss of binding to sheep erythrocytes (SRBC). Variant CTBs were characterized for the formation of immunoreactive pentamers, the ability to bind ganglioside GM(1) in vitro, and reactivity with a panel of...
Topics
- Amino Acid Sequence
- Bacterial Toxins
- Cholera Toxin
- Enterotoxins
- Epitope Mapping
- Escherichia coli Proteins
- G(M1) Ganglioside
- Genetic Variation
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Protein Binding