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Article

Biological and biochemical characterization of variant A subunits of cholera toxin constructed by site-directed mutagenesis.

Journal of bacteriology - 1 Jul 2001

Jobling M G, Holmes R K

Abstract excerpt

Cholera toxin (CT) is the prototype for the Vibrio cholerae-Escherichia coli family of heat-labile enterotoxins having an AB5 structure. By substituting amino acids in the enzymatic A subunit that are highly conserved in all members of this family, we constructed 23 variants of CT that exhibited decreased or undetectable toxicity and we characterized their biological and biochemical properties. Many variants...

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Identifiers

PMID: 11395467
DOI: 10.1128/JB.183.13.4024-4032.2001
PMCID: PMC95286

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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