Article
Expression in Escherichia coli of two mutated genes encoding the cholera toxin B subunit.
Gene - 30 Apr 1990
L'hoir C, Renard A, Martial J A
Abstract excerpt
To allow subsequent genetically mediated fusion of foreign antigens to cholera toxin B subunit (CTB), two mutated CTB encoding genes (ctxB) were constructed and overexpressed in Escherichia coli. The signal peptide coding sequence was deleted and restriction sites were created at both ends of the...
Topics
- Base Sequence
- Cholera Toxin
- Chromosomes, Bacterial
- DNA, Bacterial
- Escherichia coli
- Gene Expression
- Genes, Bacterial
- Genetic Vectors
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- Plasmids