Article
Insertional mutagenesis and immunochemical analysis of visual arrestin interaction with rhodopsin.
The Journal of biological chemistry - 5 Apr 2002
Dinculescu Astra, McDowell J Hugh, Amici Stephanie A, Dugger Donald R, Richards Nigel, Hargrave Paul A, Smith W Clay
Abstract excerpt
Visual arrestin inactivates the phototransduction cascade by specifically binding to light-activated phosphorylated rhodopsin. This study describes the combined use of insertional mutagenesis and immunochemical approaches to probe the structural determinants of arrestin function. Recombinant arrestins with insertions of a 10-amino acid c-Myc tag (EQKLISEEDL) were expressed in yeast and characterized. When the tag...
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