Article
Recovery of rat type II cell surfactant components during primary cell culture.
American journal of physiology. Lung cellular and molecular physiology - 1 Feb 2002
Bates Sandra R, Gonzales Linda W, Tao Jian-Qin, Rueckert Peter, Ballard Philip L, Fisher Aron B
Abstract excerpt
A culture system designed to maintain the differentiated characteristics of rat type II cells based on protocols used for human fetal lung pneumocytes was investigated. Type II cells were isolated either from adult rats with elastase (adult type II cells) or from young rats (4-11 days postnatal) with collagenase and trypsin (young type II cells) and were incubated with dexamethasone (Dex, 10 nM) and cAMP (0.1...
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