Article
Primary cell culture of human type II pneumonocytes: maintenance of a differentiated phenotype and transfection with recombinant adenoviruses.
American journal of respiratory cell and molecular biology - 1 Dec 1997
Alcorn J L, Smith M E, Smith J F, Margraf L R, Mendelson C R
Abstract excerpt
Studies of the regulation of surfactant lipoprotein metabolism and secretion and surfactant protein gene expression have been hampered by the lack of a cell culture system in which the phenotypic properties of type II cells are maintained. We have developed a primary culture system that facilitat...
Topics
- Adenoviridae
- Bucladesine
- Cell Differentiation
- Cells, Cultured
- Culture Media
- Dexamethasone
- Gene Expression Regulation
- Humans
- Lung
- Organ Culture Techniques
- Phenotype
- Proteolipids
- Pulmonary Surfactant-Associated Protein A
- Pulmonary Surfactant-Associated Proteins
- Pulmonary Surfactants
- Recombination, Genetic
- Transfection