Maintenance of the mouse type II cell phenotype in vitro.

doi:10.1152/ajplung.00302.2001

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Article

Maintenance of the mouse type II cell phenotype in vitro.

American journal of physiology. Lung cellular and molecular physiology - 1 Aug 2002

Rice Ward R, Conkright Juliana J, Na Cheng-Lun, Ikegami Machiko, Shannon John M, Weaver Timothy E

Abstract excerpt

The purpose of this study was to identify culture conditions for maintenance of isolated mouse type II cells with intact surfactant protein (SP) and phospholipid production. Type II cells were isolated from 6-wk-old mice and cultured on Matrigel matrix-rat tail collagen (70:30 vol/vol) in bronchial epithelial cell growth medium minus hydrocortisone plus 5% charcoal-stripped FBS and 10 ng/ml keratinocyte growth...

Topics

Animals
Cells, Cultured
Epithelial Cells
Female
Mice
Mice, Inbred C57BL
Phenotype
Phospholipids
Pulmonary Alveoli
Pulmonary Surfactants
Rats
Surface Properties
Time Factors

Source

Open in PubMed

Identifiers

PMID: 12114186
DOI: 10.1152/ajplung.00302.2001

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