Maintenance of differentiated phenotype by mouse type 2 pneumocytes in serum-free primary culture.

doi:10.3109/01902149509031746

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Article

Maintenance of differentiated phenotype by mouse type 2 pneumocytes in serum-free primary culture.

Experimental lung research - 1 Jan 2000

Kumar R K, Li W, O'Grady R

Abstract excerpt

An improved method has been developed for separation of an enriched population of mouse type 2 pneumocytes, based on differential adherence and size fractionation of cells dissociated with trypsin. These cells were successfully maintained in primary culture in serum-free medium MCDB 201 supplemen...

Topics

Animals
Cell Differentiation
Cell Separation
Cells, Cultured
Culture Media
Cytological Techniques
DNA
Extracellular Matrix
Immunohistochemistry
Mice
Microscopy, Electron
Phenotype
Proteolipids
Pulmonary Alveoli
Pulmonary Surfactant-Associated Proteins
Pulmonary Surfactants

Source

Open in PubMed

Identifiers

PMID: 7729380
DOI: 10.3109/01902149509031746

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7729380-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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