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Article

Maintenance of differentiated phenotype by mouse type 2 pneumocytes in serum-free primary culture.

Experimental lung research - 1 Jan 2000

Kumar R K, Li W, O'Grady R

Abstract excerpt

An improved method has been developed for separation of an enriched population of mouse type 2 pneumocytes, based on differential adherence and size fractionation of cells dissociated with trypsin. These cells were successfully maintained in primary culture in serum-free medium MCDB 201 supplemented with albumin, transferrin, and lipids. Whereas type 2 pneumocytes in serum-supplemented culture undergo phenotypic...

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PMID: 7729380
DOI: 10.3109/01902149509031746

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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