Article
[Isolation and characterization of human alveolar type II cells and phenotypes maintaining study].
Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue - 1 Jul 2012
Wu Song-li, Mao Pu, Fu Wei, Mo Hong-ying, He Wei-qun, Liu Xiao-qing, Li Yi-min
Abstract excerpt
OBJECTIVE: To establish a method of isolate, purify, primary culture and identify human alveolar type II cells (AT II ) in vitro, as well as its possible maintaining phenotype characteristics. METHODS: The marginal lung tissue was collected. AT II cells were isolated with trypsin and elastase, purified by a series of steps, such as, cell sieve filtration, differential adhesion, gradient separation and anti-CD14...
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