Article
Induction of T --> G and T --> A transversions by 5-formyluracil in mammalian cells.
Mutation research - 15 Jan 2002
Kamiya Hiroyuki, Murata-Kamiya Naoko, Karino Naoko, Ueno Yoshihito, Matsuda Akira, Kasai Hiroshi
Abstract excerpt
Oxidatively damaged thymine, 5-formyluracil (5-fU), was incorporated into a predetermined site of double-stranded shuttle vectors. The nucleotide sequences in which the modified base was incorporated were 5'-CFTAAG-3' and 5'-CTFAAG-3' (F represents 5-fU), the recognition site for the restriction enzyme AflII (5'-CTTAAG-3'). The 5-fU was incorporated into a template strand of either the leading or lagging strand...
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