Article
SNP genotyping by multiplexed solid-phase amplification and fluorescent minisequencing.
Genome research - 1 Nov 2001
Shapero M H, Leuther K K, Nguyen A, Scott M, Jones K W
Abstract excerpt
The emerging role of single-nucleotide polymorphisms (SNPs) in clinical association and pharmacogenetic studies has created a need for high-throughput genotyping technologies. We describe a novel method for multiplexed genotyping of SNPs that employs PCR amplification on microspheres. Oligonucleotide PCR primers were designed for each polymorphic locus such that one of the primers contained a recognition site for...
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