Article
Observation of covalent intermediates in an enzyme mechanism at atomic resolution.
Science (New York, N.Y.) - 12 Oct 2001
Heine A, DeSantis G, Luz J G, Mitchell M, Wong C H, Wilson I A
Abstract excerpt
In classical enzymology, intermediates and transition states in a catalytic mechanism are usually inferred from a series of biochemical experiments. Here, we derive an enzyme mechanism from true atomic-resolution x-ray structures of reaction intermediates. Two ultra-high resolution structures of wild-type and mutant d-2-deoxyribose-5-phosphate (DRP) aldolase complexes with DRP at 1.05 and 1.10 angstroms...
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