Article

A Unified Mechanism Reveals the Evolutionary Origin of Enoyl Isomerases

2025-08-26

Abstract excerpt

Enoyl isomerases (EIs) and dehydratases (DHs) are critical for diversifying polyketide natural products; however, the underlying mechanism remains controversial. Through a combination of 1 H NMR, solvent isotope effect (SIE) analyses, and mutagenesis applied to two bifunctional dehydratase/enoyl isomerases (DH/EIs), a “His-solo” monofunctional EI, and an engineered “Asp-solo” monofunctional EI, we uncovered a unif...

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Literature Corpus work: 12c3efc5-a782-5884-b879-018d9ec3a75c
DOI: 10.1021/acscatal.5c05457

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