Glutamate 350 Plays an Essential Role in Conformational Gating of Long-Range Radical Transport in Escherichia coli Class Ia Ribonucleotide Reductase.

doi:10.1021/acs.biochem.6b01145

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Glutamate 350 Plays an Essential Role in Conformational Gating of Long-Range Radical Transport in Escherichia coli Class Ia Ribonucleotide Reductase.

Biochemistry - 14 Feb 2017

Ravichandran Kanchana, Minnihan Ellen C, Lin Qinghui, Yokoyama Kenichi, Taguchi Alexander T, Shao Jimin, Nocera Daniel G, Stubbe JoAnne

Abstract excerpt

Escherichia coli class Ia ribonucleotide reductase (RNR) is composed of two subunits that form an active α2β2 complex. The nucleoside diphosphate substrates (NDP) are reduced in α2, 35 Å from the essential diferric-tyrosyl radical (Y122•) cofactor in β2. The Y122•-mediated oxidation of C439 in α2 occurs by a pathway (Y122 ⇆ [W48] ⇆ Y356 in β2 to Y731 ⇆ Y730 ⇆ C439 in α2) across the α/β interface. The absence of...

Topics

Adenosine Triphosphate
Amino Acid Substitution
Apoenzymes
Binding, Competitive
Biocatalysis
Cytidine Diphosphate
Electron Spin Resonance Spectroscopy
Electron Transport
Escherichia coli Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Free Radicals

Glutamic Acid

Hydrogen-Ion Concentration

Kinetics

Models, Molecular

Mutagenesis, Site-Directed

Mutation

Oxidation-Reduction

Protein Conformation

Source

Open in PubMed Open full text

Identifiers

PMID: 28103007
DOI: 10.1021/acs.biochem.6b01145
PMCID: PMC5689411

Genes

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Glutamate 350 Plays an Essential Role in Conformational Gating of Long-Range Radical Transport in Escherichia coli Class Ia Ribonucleotide Reductase.PMID 28103007Read ↗

Gated Proton Release during Radical Transfer at the Subunit Interface of Ribonucleotide Reductase.Semantic proximity Read ↗

Conformationally Dynamic Radical Transfer within Ribonucleotide Reductase.Semantic proximity Read ↗

Investigation of in vivo roles of the C-terminal tails of the small subunit (ββ') of Saccharomyces cerevisiae ribonucleotide reductase: contribution to cofactor formation and intersubunit association within the active holoenzyme.Semantic proximity Read ↗

Electron transfer associated with oxygen activation in the B2 protein of ribonucleotide reductase from Escherichia coli.Semantic proximity Read ↗

Mutations in the R2 subunit of ribonucleotide reductase that confer resistance to hydroxyurea.Semantic proximity Read ↗

Electron injection through a specific pathway determines the outcome of oxygen activation at the diiron cluster in the F208Y mutant of Escherichia coli ribonucleotide reductase protein R2.Semantic proximity Read ↗

Structural and functional characterization of two mutated R2 proteins of Escherichia coli ribonucleotide reductase.Semantic proximity Read ↗

Dioxygen is the source of the mu-oxo bridge in iron ribonucleotide reductase.Semantic proximity Read ↗

Metal-independent ribonucleotide reduction powered by a DOPA radical in Mycoplasma pathogensSemantic proximity Read ↗

Interaction of C225SR1 mutant subunit of ribonucleotide reductase with R2 and nucleoside diphosphates: tales of a suicidal enzyme.Semantic proximity Read ↗

In vivo assay for low-activity mutant forms of Escherichia coli ribonucleotide reductase.Semantic proximity Read ↗

Inactivation of ribonucleotide reductase by (E)-2'-fluoromethylene-2'-deoxycytidine 5'-diphosphate: a paradigm for nucleotide mechanism-based inhibitors.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.