Article
Altered conformational sampling along an evolutionary trajectory changes the catalytic activity of an enzyme.
Nature communications - 23 Nov 2020
Kaczmarski Joe A, Mahawaththa Mithun C, Feintuch Akiva, Clifton Ben E, Adams Luke A, Goldfarb Daniella, Otting Gottfried, Jackson Colin J
Abstract excerpt
Several enzymes are known to have evolved from non-catalytic proteins such as solute-binding proteins (SBPs). Although attention has been focused on how a binding site can evolve to become catalytic, an equally important question is: how do the structural dynamics of a binding protein change as it becomes an efficient enzyme? Here we performed a variety of experiments, including propargyl-DO3A-Gd(III) tagging and...
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