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Article

Structure and mechanism of the glycyl radical enzyme pyruvate formate-lyase.

Nature structural biology - 1 Oct 1999

Becker A, Fritz-Wolf K, Kabsch W, Knappe J, Schultz S, Volker Wagner A F

Abstract excerpt

Pyruvate formate-lyase (PFL) from Escherichia coli uses a radical mechanism to reversibly cleave the C1-C2 bond of pyruvate using the Gly 734 radical and two cysteine residues (Cys 418, Cys 419). We have determined by X-ray crystallography the structures of PFL (non-radical form), its complex with the substrate analog oxamate, and the C418A,C419A double mutant. The atomic model (a dimer of 759-residue monomers)...

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PMID: 10504733
DOI: 10.1038/13341

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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