Article
Phage display selection of hairpin loop soyacystatin variants that mediate high affinity inhibition of a cysteine proteinase.
The Plant journal : for cell and molecular biology - 1 Sept 2001
Koiwa H, D'Urzo M P, Assfalg-Machleidt I, Zhu-Salzman K, Shade R E, An H, Murdock L L, Machleidt W, Bressan R A, Hasegawa P M
Abstract excerpt
Two hairpin-loop domains in cystatin family proteinase inhibitors form an interface surface region that slots into the active site cleft of papain-like cysteine proteinases, and determine binding affinity. The slot region surface architecture of the soybean cysteine proteinase inhibitor (soyacystatin N, scN) was engineered using techniques of in vitro molecular evolution to define residues that facilitate...
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