Article
Protease inhibitor display M13 phage: selection of high-affinity neutrophil elastase inhibitors.
Gene - 2 Nov 1992
Roberts B L, Markland W, Siranosian K, Saxena M J, Guterman S K, Ladner R C
Abstract excerpt
We report display of the complete protease inhibitor (Kunitz) domain, BPTI, on the surface of bacteriophage M13 as a fusion to the gene III product. Phage that display BPTI bind specifically to anti-BPTI antibodies, trypsin and anhydrotrypsin. A point mutation of BPTI [Lys15-->Leu(K15L)] alters t...
Topics
- Amino Acid Sequence
- Aprotinin
- Bacteriophage M13
- Base Sequence
- DNA, Viral
- Hydrogen-Ion Concentration
- Leukocyte Elastase
- Membrane Proteins
- Molecular Sequence Data
- Mutagenesis
- Pancreatic Elastase
- Phenotype