Article
Protease inhibitor display M13 phage: selection of high-affinity neutrophil elastase inhibitors.
Gene - 2 Nov 1992
Roberts B L, Markland W, Siranosian K, Saxena M J, Guterman S K, Ladner R C
Abstract excerpt
We report display of the complete protease inhibitor (Kunitz) domain, BPTI, on the surface of bacteriophage M13 as a fusion to the gene III product. Phage that display BPTI bind specifically to anti-BPTI antibodies, trypsin and anhydrotrypsin. A point mutation of BPTI [Lys15-->Leu(K15L)] alters the binding specificity of fusion phage such that a human neutrophil elastase-binding phenotype is conferred while a...
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