Article
A homogeneous europium cryptate-based assay for the diagnosis of mutations by time-resolved fluorescence resonance energy transfer.
Nucleic acids research - 15 Jul 2001
Lopez-Crapez E, Bazin H, Andre E, Noletti J, Grenier J, Mathis G
Abstract excerpt
Oligonucleotide ligation assay (OLA) is considered to be a very useful methodology for the detection and characterization of mutations, particularly for clinical purposes. The fluorescence resonance energy transfer between a fluorescent donor and a suitable fluorophore as acceptor has been applied in the past to several scientific fields. This technique is well adapted to nucleic acid analysis such as DNA...
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