Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A new label technology for the detection of specific polymerase chain reaction products in a closed tube.

Nucleic acids research - 15 Apr 2000

Nurmi J, Ylikoski A, Soukka T, Karp M, Lövgren T

Abstract excerpt

A novel signal generation principle suitable for real time and end-point detection of specific PCR products in a closed tube is described. Linear DNA probes were labeled at their 5'-ends with a stable, fluorescent terbium chelate. The fluorescence intensity of this chelate is lower when it is coupled to single-stranded DNA than when the chelate is free in solution. The synthesized probes were used in the real...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Chelating Agents
Share to DNA-Directed DNA Polymerase
Share to DNA Probes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Feasibility Studies

Share to Fluorescent Dyes

Share to Fluorometry

Share to Nucleic Acids

Share to Prostate-Specific Antigen

Source

Open in PubMed Open full text

Identifiers

PMID: 10734205
DOI: 10.1093/nar/28.8.e28
PMCID: PMC102832

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A new label technology for the detection of specific polymerase chain reaction products in a closed tube.PMID 10734205Read ↗

Real-time PCR in the microbiology laboratory.Semantic proximity Read ↗

Universal molecular beacon-based tracer system for real-time polymerase chain reaction.Semantic proximity Read ↗

Detection of single nucleotide variations by a hybridization proximity assay based on molecular beacons and luminescence resonance energy transfer.Semantic proximity Read ↗

Shedding light on health and disease using molecular beacons.Semantic proximity Read ↗

Combination of fluorescence color and melting temperature as a two-dimensional label for homogeneous multiplex PCR detection.Semantic proximity Read ↗

Universal quenching probe system: flexible, specific, and cost-effective real-time polymerase chain reaction method.Semantic proximity Read ↗

Real-time closed tube single nucleotide polymorphism (SNP) quantification in pooled samples by quencher extension (QEXT).Semantic proximity Read ↗

Template-directed dye-terminator incorporation (TDI) assay: a homogeneous DNA diagnostic method based on fluorescence resonance energy transfer.Semantic proximity Read ↗

A label-free signal amplification assay for DNA detection based on exonuclease III and nucleic acid dye SYBR Green I.Semantic proximity Read ↗

Multiplex real-time single nucleotide polymorphism detection and quantification by quencher extension.Semantic proximity Read ↗

An exciplex-based, target-assembled fluorescence system with inherently low background to probe for specific nucleic acid sequences.Semantic proximity Read ↗

Sensitive fluorescence method for detecting DNA-ligation amplification products.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.