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Article

A separation-free assay for the detection of mutations: combination of homogeneous time-resolved fluorescence and minisequencing.

Human mutation - 1 May 2005

Lopez-Crapez Evelyne, Bazin Hervé, Chevalier Julien, Trinquet Eric, Grenier Jean, Mathis Gérard

Abstract excerpt

Single nucleotide primer extension reaction has been widely used in DNA testing, and several detection methods based on this core allelic discrimination have been developed. Most of the reported formats are based on a two step protocol involving first, a liquid phase extension reaction, then a physical separation process (chromatography, electrophoresis, capture on solid support, mass spectrometry). Here we...

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Identifiers

PMID: 15832307
DOI: 10.1002/humu.20162

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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