Article
Kir6.2 channel gating by intracellular protons: subunit stoichiometry for ligand binding and channel gating.
The Journal of membrane biology - 1 Jan 2006
Wang Runping, Su Junda, Zhang Xiaoli, Shi Yun, Cui Ningren, Onyebuchi Vivian A, Jiang Chun
Abstract excerpt
The adenosine triphosphate-sensitive K(+) (K(ATP)) channels are gated by several metabolites, whereas the gating mechanism remains unclear. Kir6.2, a pore-forming subunit of the K(ATP) channels, has all machineries for ligand binding and channel gating. In Kir6.2, His175 is the protonation site and Thr71 and Cys166 are involved in channel gating. Here, we show how individual subunits act in proton binding and...
Topics
- Animals
- Cytoplasm
- Female
- Ion Channel Gating
- Ligands
- Mice
- Mutation
- Oocytes
- Patch-Clamp Techniques
- Potassium Channels, Inwardly Rectifying
- Protein Conformation