Article
3' splice site recognition in nematode trans-splicing involves enhancer-dependent recruitment of U2 snRNP.
RNA (New York, N.Y.) - 1 Jun 2001
Romfo C M, Maroney P A, Wu S, Nilsen T W
Abstract excerpt
Trans-splicing requires that 5' and 3' splice sites be independently recognized. Here, we have used mutational analyses and a sensitive nuclease protection assay to determine the mechanism of trans-3' splice site recognition in vitro. Efficient recognition of the 3' splice site is dependent upon both the sequence of the 3' splice site itself and enhancer elements located in the 3' exon. We show that the presence...
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