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Article

A high-throughput pH indicator assay for screening glycosyltransferase saturation mutagenesis libraries.

Analytical biochemistry - 1 Jul 2008

Persson Mattias, Palcic Monica M

Abstract excerpt

Protein engineering using directed evolution or saturation mutagenesis at hot spots is often used to improve enzyme properties such as their substrate selectivity or stability. This requires access to robust high-throughput assays to facilitate the analysis of enzyme libraries. However, relatively few studies on directed evolution or saturation mutagenesis of glycosyltransferases have been reported in part due to...

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Identifiers

PMID: 18405657
DOI: 10.1016/j.ab.2008.03.006

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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