Article
Structure-based engineering of E. coli galactokinase as a first step toward in vivo glycorandomization.
Chemistry & biology - 1 Jun 2005
Yang Jie, Fu Xun, Liao Jianchun, Liu Lesley, Thorson Jon S
Abstract excerpt
In vitro glycorandomization is a rapid chemoenzymatic strategy to diversify complex natural product scaffolds. The glycorandomization sugar activation pathway is dependent upon the efficient construction of diverse sugar-1-phosphate libraries. In the context of the previously evolved GalK Y371H "gatekeeper" mutation, the active site M173L mutation described herein presents a kinase with remarkably broadened...
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