Article
Posttranscriptional regulation of human ADH5/FDH and Myf6 gene expression by upstream AUG codons.
Archives of biochemistry and biophysics - 15 Feb 2001
Kwon H S, Lee D K, Lee J J, Edenberg H J, Ahn Y H, Hur M W
Abstract excerpt
Upstream open-reading frames are unusual in mammalian mRNAs. The 5' untranslated region of ADH5 mRNA contains an upstream open-reading frame (uORF) with two possible AUG start codons. Myf6 mRNA contains three tandem AUG repeats at the translation start site, a rare feature. Mutation at one or both of the upstream AUG codons in the ADH5 mRNA increased gene expression twofold in CV-1, NIH/3T3, HeLa, and SL2 cells....
Topics
- 3T3 Cells
- 5' Untranslated Regions
- Aldehyde Oxidoreductases
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line
- Codon, Initiator
- Down-Regulation
- Gene Expression Regulation
- HeLa Cells